A.N.P. an Asepta Exclusive

A FUNDAMENTAL DISCOVERY IN HAIR AND NAIL CARE

- The Ecrinal Range of treatments for hair loss is based on A.N.P.®

• A.N.P.® is a natural substance rich in TRICHOLIPIDS®
• A.N.P.® acts on the dermal papilla by activating the production of SH groups, indicative of active metabolism vital to the synthesis of methionine and cysteine, the essential amino acid constituents of hair, eyelashes, eyebrows, and nails.
• A.N.P.® is an exclusive and patented active ingredient in ECRINAL preparations from Asepta.

- RESULT: STRONGER HAIR AND NAILS, FASTER GROWTH

DEFINITION

ANP® (horse mane extract) is an exclusive and internationally patented ingredient used by Laboratoires Asepta in the Ecrinal Range for hair and nail care.
ANP® is an active natural ingredient rich in omega 3 fatty acids. It stimulates the production of keratin.
Different pharmaceutical forms of this active ingredient have been used in the Ecrinal Range to allow the full benefits of its properties to be made use of in the nail care range and the treatment of hair loss, hair maintenance and health including eyelashes and eyebrows.

DISCOVERY OF HORSEMANE (ANP®)

In the majority of mammals, the hair bulb is located in a layer of a very particular type of oily tissue. The permanent presence of this oily mass suggests that it is necessary for hair growth. These observations were made in animals with manes such as lions, giraffes, horses, etc.

The discovery that this oily substance has a highly beneficial effect on the phaneres (keratinised parts of the human body: nails, eyelashes and hair) was made in a hospital. The spectacular results obtained with nails led scientists to study this new active substance very carefully.

A team of French hospital pharmacists, in collaboration with the ASEPTA Laboratories, have developed a method for separating and purifying Horsemane Tricholipids or A.N.P.® (Natural Hair and Nail Activator) exclusively used by the ASEPTA Laboratories in our ECRINAL ANP® nail, eyelash and hair range.

ANP® ACTIVITY

Histochemical studies have shown that Tricholipids® mobilise an active form of sulphur, the thiol or SH groups. This points to an active growth and regeneration metabolic process, vital to active biosynthesis of cysteine and methionine, the sulphurated amino acids which are vital constituents of nails and hair.
Pharmacological studies have shown that certain specific enzyme activities, such as phosphatase activity in tissue located at the base of hairs, increase after treatment with Tricholipids. Tricholipids penetrate very quickly which means faster tissue mobilisation and stimulation of bulb activity leading to hair growth.
A.N.P. contains specific substances and a high concentration of essential fatty acids including linoleic acid of the omega 3 family. Its intense biodynamic stimulation of hair and nail growth has been confirmed by pharmacodynamic studies and a clinical study.

Results of Clinical Studies on ANP 40

Treatment consisted of vials containing 40% ANP®

STUDY ONE

Study on the Anti-Hair-Loss Effect After a 2 Month Treatment \
and On the Hair Re-Growth Effect After a 4 Month Treatment

Study conducted under dermatological supervision by:

• Professor Jacques GUILAINE Head of Dermatology (Beaujon Hospital), expert with the Ministry of Health
• Professor Bernard KALIS Head of Dermatology (Reims University Hospital Centre) Expert with the Ministry of Health

The effect of the product ECRINAL A.N.P. 40® VIALS was evaluated in 71 men and 22 women (total: 93 patients). It is important to note that the cause of hair loss in men and women can be very different. This means the benefits of the treatment in the two sexes can be understood better.

Number of applications:

• first month: 3 to 5 ampoules per week
• 3 following months: 1 to 2 ampoules per week

Results

Effect On Hair Loss

After 2 Months of Treatment, hair loss stopped or was considerably reduced in 85% of Cases

Effect On Hair Re-Growth

After 4 Months of hair re growth treatment, there was obvious hair re-growth in 67% of cases, while 11% of cases showed the growth of baby hair.

Effect On Excess Seborrhoea

(Study conducted n people with hyperseborrhoea, a common cause of alopecia in men) After 4 Months of Treatment, a distinct improvement in seborrhoea was noted in 91% of cases.

STUDY TWO

Effect Of Ecrinal ANP40® Treatment On Hair Loss
A 2 Month Study of 93 Cases

Results

Good results, especially for male seborrhoeic alopecia,
often the most disabling and hardest to treat.

	HAIR LOSS STOPS OR 3/4 DECREASE	HAIR LOSS HALVED	NO ACTIVITY
IN WOMEN	14	5	5
IN MEN	51	9	9
TOTAL	65	14	14

STUDY THREE

Effect Of Ecrinal ANP40® On Hair Re-Growth
A 4 Month Study of 64 Cases

Results

	VERY OBVIOUS RE-GROWTH OR 3/4 DECREASE	GROWTH OF 'BABY HAIR'	NO ACTIVITY
IN WOMEN	9	2	3
IN MEN	34	5	11
TOTAL	43	7	14

STUDY FOUR

Effect Of Ecrinal ANP40® Treatment On Seborrhoea
A 4 Month Study of 56 Cases

A reduction in seborrhoeic flow after 2 months, and an improvement after 4 months.

Results

	VERY OBVIOUS IMPROVEMENT	MODERATE IMPROVEMENT	NO ACTIVITY
IN WOMEN	6	4	1
IN MEN	29	12	4
TOTAL	35	16	5

STUDY FIVE

General Effect On Scalp and Hair Condition A 4 Month Study of 51 Cases

Results

	VERY GOOD RESULTS	AVERAGE RESULTS	NO CHANGE
IN WOMEN	9	2	0
IN MEN	36	3	1
TOTAL	45	5	1

STUDY SIX

Ecrinal ANP40® Product Tolerance - A Study of 93 Cases

Results

Ecrinal ANP40® treatment has been shown to be very well tolerated
during periods of 2 months and longer.

	VERY GOOD AND GOOD	AVERAGE IMPOVEMENT
IN MEN AND WOMEN	92% of cases	8% of cases

STUDY SEVEN

Study of the Anti-Hair-Loss and Redensifying Effect
Of Ecrinal ANP40® After 8 Weeks of Treatment

• Study conducted under dermatological supervision
• Number of volunteers: 18 (men and women)
• Inclusion criteria for volunteers: Proportion of hair in the telogenic phase equal to or greater than 15% for men and equal to or greater than 10% for women
• Duration of the test: 2 Months
• Number of applications: 1st month: 1 ampoule applied every 2 days, 2nd month: 1 ampoule applied every 3 days
• Method of application: The content of one ampoule applied to the scalp, masaged in well, left for 30 minutes, and hair then washed with Ecrinal ANP Shampoo
• Evaluation criteria

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• Subjective evaluation of product efficacy
• Determination of pilar formula (pilar density, proportion of hair in the anagenic and telogenic phases) before and after 8 weeks of treatment using the videotrichogram method and analysis of results by Cont' hair® software.

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Parameters Assessed:

• Capillary density: total number of hairs per square cm
• Number of hairs in the anagenic phase (growth phase)
• Number and proportion (%) of hairs in the telogenic phase (no growth)

Results

Subjective Evaluation

Results

Determination of Pilar Formula

A significant anti-hair loss and redensifying effect is observed by the vounteers themselves:

• Anti-Hair Loss Effect: Decrease in the proportion (-3%*) and density of hair in the telogenic phase (-13%*)
• Redensifying Effect: Increase in the density of hair in the anagenic phase (+7%**)